Keywords: DNA Nanoball Adapter Ligation.png en The Adapter sequences AD1L and AD1R are ligated to the size selected DNA The adapters complement each other and the DNA is circularized A restriction enzyme is added that cleaves the DNA AD2L and AD2R are ligated the DNA is circularized and a restriction enzyme cleaves the DNA AD3L and AD3R are added the DNA is circularized and a type III restriction enzyme is added This cleaves out a 300 - 400bp piece of DNA and AD4 replaces it to complete the circular adapter ligated template DNA own Suspencewl Cc-zero DNA helix-structures Biotechnology |