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| Keywords: Tlc sequence.png Tlc sequence svg A mixture is added to the base line 1 shown as purple spots The TLC plate is palced in a solvent tank and the solvent moves up the plate A lid ensures that the atmosphere in the tank is saturated with solvent As the solvent moves up the plate the purple mixture separates into a fast moving red spot and a slower moving blue spot The R<sub>f</sub> of each spot is worked out by measuring the distance the spot moved relative to the distance the solvent moved i e for the red spot the R<sub>f</sub> is 1 to 3 / 1 to 4 and for the blue spot the R<sub>f</sub> is 1 to 2 / 1 to 4 The R<sub>f</sub> of the red spot is larger meaning that it has moved further along the plate in that solvent than has the blue spot In this case it can be seen that the purple spot can be separated easily into 2 spots using the light blue solvent wiki 06/12/09 Quantockgoblin Thin layer chromatography | ||||
