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| Keywords: Yoctoreactor library assembly wiki3.JPG en Figure 6 Stepwise assembly of a DEL library using YoctoReactor technology A 3-way reactor is shown here a Position 1 P1 and P2 BB are brought into close proximity and undergo a chemical reaction in the presence of a helper oligonucleotide in P3 b The structure is purified by polyacrylamide gel electrophoresis PAGE the P1 and P2 DNA is ligated and the P2 linker is cleaved c P3 BB is annealed to the P1-P2 ligation product from step b and a chemical reaction between P2 and P3 BBs takes place d The reaction product is purified by PAGE the DNA is ligated and P3 linker is cleaved yielding a compound OOO covalently attached to the folded yR e The yR is dismantled by primer extension yielding a double-stranded disply product exposing the reaction product for selection and molecular evolution own Viptrh 2010-05-21 Biochemistry | ||||
